The CARS contrast-generation mechanism is based on the chemical properties the sample: a couple of incoming photons (“pump” and “Stokes”) resonantly and coherently excite the vibrational modes of a molecular bond of interest (the symmetric and antisymmetric stretch modes
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چکیده
Western Blot Membranes were immunoblotted with the following primary antibodies: rabbit polyclonal anti-Lmp7 (1:2000; AbD Serotec); rabbit polyclonal anti-β2M (1:1000; Proteintech); mouse anti β-actin (1:30000; Chemicon); mouse anti importin-β; (1:5000; Millipore); mouse anti-acetylated tubulin (1:1000; Millipore); mouse anti β-tubulin (1:1000; Millipore); rabbit anti-ERK (K23, 1:1000; Santa Cruz Biotech); mouse antiphospho-ERK (E4, 1:1000; Santa Cruz Biotech); rabbit anti-NF200 (1:1000; Sigma-Aldrich); mouse antiGFAP (1:2000; Millipore); rabbit polyclonal anti-C3 (H300, 1:100; Santa Cruz) followed by HRPconjugated secondary antibodies (Santa Cruz) and developed by Luminata Forte Western Chemiluminescent HRP Substrate (Millipore) on the Chemi-Doc XRS system (Bio-Rad).
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تاریخ انتشار 2016